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Measurement and Evaluation of Axis Straightness of Rotary Forgings using Photogrammetry and Image Analysis
Zatočilová, Aneta ; Druckmüller, Miloslav (referee) ; Matula, Pavel (referee) ; Morovič,, Ladislav (referee) ; Brandejs, Jan (advisor)
The thesis deals with methodology proposal for shape and dimension measurement of rotationally symmetric forgings. Most of available non-contact systems are not possible to use for this purpose, mainly due to high temperatures and large dimensions of forgings. Only a few commercial systems which are designed for this specific measurement are currently in the market. These systems are based on a principle of evaluating time response of posted modulated signal. However, scientific literature shows also other approaches to the measurement, which are based on principle of triangulation and methods of image analysis. Advantage of systems based on these principles should be especially measurement speed and significantly lower price. The paper describes current state of research and development in this area and proposes a new approach which is based on passive photogrammetry and image analysis. The core of the work is devoted to the design of partial methods and their implementation in the Matlab programming language. Functionality of the methodology and the software has been verified by measuring cylindrical tubes of small dimensions in laboratory conditions. The designed methods were discussed and evaluated based on statistical analysis of the measurement results, and further steps to increase the accuracy and reduce the spread of results were suggested. Optimization and further development of this methodology could lead to the design a professional measuring system intended for this specific application.
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Optical Measurement of Electromechanical Characteristics of Heart Cells
Čmiel, Vratislav ; Križanová,, Olga (referee) ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
Dissertation is focused on the application of optical measurement methods using techniques of optical microscopy and fluorescence microscopy in measurements of electromechanical characteristics of isolated cardiac cells and clusters of differentiated cardiomyocytes. The first proposed method uses a practical combination of fluorescence microscopy equipped with fluorescent fast and high-resolution camera and atomic force microscopy for simultaneous measurement of calcium transients and contraction of cardiomyocyte clusters. The signals obtained undergoes filtration, processing and analysis. Result function parameters obtained by analyzing signals after application of caffeine are evaluated by comparison with functional parameters obtained during the control measurement. The second proposed method is applied to the cardiomyocyte clusters for the purpose of cardiomyocyte contraction signals measurement. The signals obtained by optical methods are analyzed and compared with the reference signal obtained using atomic force microscopy. Optical measurement method of cell contractins based on detection of cell ends using adjusting of microscopy images by re-sharpening and fluorescence method for cardiomyocyte contractions measurements were designed to increase realiability in simultaneous measurement of cell contractions simultaneously with calcium transients in isolated cardiomyocytes experiments.
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Optical Measurement of Electromechanical Characteristics of Heart Cells
Čmiel, Vratislav ; Križanová,, Olga (referee) ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
Dissertation is focused on the application of optical measurement methods using techniques of optical microscopy and fluorescence microscopy in measurements of electromechanical characteristics of isolated cardiac cells and clusters of differentiated cardiomyocytes. The first proposed method uses a practical combination of fluorescence microscopy equipped with fluorescent fast and high-resolution camera and atomic force microscopy for simultaneous measurement of calcium transients and contraction of cardiomyocyte clusters. The signals obtained undergoes filtration, processing and analysis. Result function parameters obtained by analyzing signals after application of caffeine are evaluated by comparison with functional parameters obtained during the control measurement. The second proposed method is applied to the cardiomyocyte clusters for the purpose of cardiomyocyte contraction signals measurement. The signals obtained by optical methods are analyzed and compared with the reference signal obtained using atomic force microscopy. Optical measurement method of cell contractins based on detection of cell ends using adjusting of microscopy images by re-sharpening and fluorescence method for cardiomyocyte contractions measurements were designed to increase realiability in simultaneous measurement of cell contractions simultaneously with calcium transients in isolated cardiomyocytes experiments.
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Measurement and Evaluation of Axis Straightness of Rotary Forgings using Photogrammetry and Image Analysis
Zatočilová, Aneta ; Druckmüller, Miloslav (referee) ; Matula, Pavel (referee) ; Morovič,, Ladislav (referee) ; Brandejs, Jan (advisor)
The thesis deals with methodology proposal for shape and dimension measurement of rotationally symmetric forgings. Most of available non-contact systems are not possible to use for this purpose, mainly due to high temperatures and large dimensions of forgings. Only a few commercial systems which are designed for this specific measurement are currently in the market. These systems are based on a principle of evaluating time response of posted modulated signal. However, scientific literature shows also other approaches to the measurement, which are based on principle of triangulation and methods of image analysis. Advantage of systems based on these principles should be especially measurement speed and significantly lower price. The paper describes current state of research and development in this area and proposes a new approach which is based on passive photogrammetry and image analysis. The core of the work is devoted to the design of partial methods and their implementation in the Matlab programming language. Functionality of the methodology and the software has been verified by measuring cylindrical tubes of small dimensions in laboratory conditions. The designed methods were discussed and evaluated based on statistical analysis of the measurement results, and further steps to increase the accuracy and reduce the spread of results were suggested. Optimization and further development of this methodology could lead to the design a professional measuring system intended for this specific application.
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